MAGED1 Monoclonal Antibody

$Lane 1: Jurkat cell lysates; Lane 2: 293 cell lysatess probed with MAGED1 Monoclonal Antibody, Unconjugated (bsm-51582M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Jurkat cell lysates; Lane 2: 293 cell lysatess probed with MAGED1 Monoclonal Antibody, Unconjugated (bsm-51582M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51582m
Product Name	MAGED1 Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human MAGED1
Immunogen Range	50-200/778
Clonality	Monoclonal
Clone #	C5D3
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9500
Swiss Prot	Q9Y5V3
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	DLXIN 1; MAGD1_HUMAN; MAGE D1 antigen; MAGE tumor antigen CCF; MAGE-D1 antigen; Melanoma antigen family D 1; Melanoma associated antigen D1; Melanoma-associated antigen D1; Neurotrophin receptor interacting MAGE homolog; Neurotrophin receptor-interacting MAGE homolog; NRAGE; PP2250.
Background	Involved in the apoptotic response after nerve growthfactor (NGF) binding in neuronal cells. Binds NGFR/p75NTR andantagonizes its association with NTRK1/TrkA, inhibits cell cycleprogression, and facilitates NGFR-mediated apoptosis. May act as aregulator of the function of DLX family members. May regulateTP53/p53 transcriptional activity and inhibit cell proliferation.Enhances TP53 phosphorylation and accumulation. May enhanceubiquitin ligase activity of RING-type zinc finger-containing E3ubiquitin-protein ligases. Proposed to act through recruitmentand/or stabilization of the Ubl-conjugating enzyme (E2) at theE3:substrate complex.
Application Dilution
WB	=1:500-1000