TPX2 Monoclonal Antibody

$Lane 1: HT-29 cell lysates; Lane 2: K562 cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: Raji cell lysates probed with TPX2 Monoclonal Antibody, Unconjugated (bsm-51631M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: HT-29 cell lysates; Lane 2: K562 cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: Raji cell lysates probed with TPX2 Monoclonal Antibody, Unconjugated (bsm-51631M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51631m
Product Name	TPX2 Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human TPX2 between 1-531 amino acids.
Clonality	Monoclonal
Clone #	C3A12
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	22974
Swiss Prot	Q9ULW0
Subcellular location	Cytoplasm, Nucleus
Synonyms	C20ORF1; C20orf2; Chromosome 20 Open Reading Frame 1; Differentially expressed in cancerous and non-cancerous lung cells 2; Differentially expressed in cancerous and noncancerous lung cells 2; Differentially expressed in lung cells 2; Differentially expressed in lung cells; DIL 2; DIL-2; DIL2; FLS353; GD:C20orf1; HCA 519; HCA519; HCTP 4; HCTP4; Hepatocellular carcinoma associated antigen 519; Hepatocellular carcinoma-associated antigen 519; p100; Preferentially expressed in colorectal cancer; Protein FLS353; REPP 86; repp86; Restricted expression proliferation associated protein 100; Restricted expression proliferation-associated protein 100; Targeting protein for XKLP2; TPX 2; TPX2 microtubule associated homolog; TPX2 microtubule associated protein homolog; TPX2_HUMAN.
Background	Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
Application Dilution
WB	=1:500-2000