USP2 Monoclonal Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with USP2 monoclonal antibody, unconjugated (bsm-51636M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with USP2 monoclonal antibody, unconjugated (bsm-51636M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Predicted Reactivity

Monkey

Overview
Catalog #	bsm-51636m
Product Name	USP2 Monoclonal Antibody
Applications	WB
Reactivity	Human
Predicted Reactivity	Monkey
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human USP2 between 1-258 amino acids.
Clonality	Monoclonal
Clone #	K08F1
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9099
Swiss Prot	O75604
Subcellular location	Cytoplasm, Nucleus
Synonyms	Ubiquitin carboxyl-terminal hydrolase 2, 3.4.19.12, 41 kDa ubiquitin-specific protease, Deubiquitinating enzyme 2, Ubiquitin thioesterase 2, Ubiquitin-specific-processing protease 2, USP2, UBP41
Background	This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
Application Dilution
WB	1:500-2000