phospho-eIF4E (Ser209) Recombinant Antibody

$SH-SY-5Y cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with eIF4E(S209) (2B1 ) Monoclonal Antibody (bsm-52152R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.$

SH-SY-5Y cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with eIF4E(S209) (2B1 ) Monoclonal Antibody (bsm-52152R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

$Lane 1: Mouse Pancreas tissue lysates; Lane 2:Rat Lymph node tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Pancreas tissue lysates ; Lane 5: Human Hela cell lysates;Lane 6: Human 293T cell lysates ;Lane 7: Human MCF-7 cell lysates; Lane 8: Human SH-SY5Y cell lysates probed with eIF4E(S209) (2B1) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Pancreas tissue lysates; Lane 2:Rat Lymph node tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Pancreas tissue lysates ; Lane 5: Human Hela cell lysates;Lane 6: Human 293T cell lysates ;Lane 7: Human MCF-7 cell lysates; Lane 8: Human SH-SY5Y cell lysates probed with eIF4E(S209) (2B1) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-eIF4E (Ser209) Monoclonal Antibody, Unconjugated (bsm-52152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52152r
Product Name	phospho-eIF4E (Ser209) Recombinant Antibody
Applications	WB IHC-P IHC-F IF ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated Synthesised phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209
Modification Site	S209
Clonality	Recombinant
Clone #	2B1
Isotype	IgG
Concentration	Lot Dependent
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1977
Swiss Prot	P06730
Subcellular location	Cytoplasm, Nucleus
Synonyms	AUTS19; CBP; EIF4E1; EIF4EL1; EIF4F; eIF-4E; EG668879; Eif4e-ps; If4e; IF4E_HUMAN; EIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; IF4E_MOUSE; IF4E_RAT; eukaryotic translation initiation factor 4E; eIF4E (phospho-S209); p-eIF4E; phospho-eIF4E; eIF4E (phospho-Ser209)
Background	Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF
ICC/IF	1:50-100