IRF3(S386) (28C3) Monoclonal Antibody

$MCF-7 (H) cells were treated with or without Calyculin A (100nM) for 45 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-IRF3 (Ser386) monoclonal antibody, unconjugated (bsm-52170R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

MCF-7 (H) cells were treated with or without Calyculin A (100nM) for 45 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-IRF3 (Ser386) monoclonal antibody, unconjugated (bsm-52170R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-52170r
Product Name	IRF3(S386) (28C3) Monoclonal Antibody
Applications	WB ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide derived from human IRF3(S386), around 350-400aa (phospho S386).
Modification Site	S386
Clonality	Monoclonal
Clone #	28C3
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	3661
Swiss Prot	Q14653
Synonyms	Bone Morphogenic Protein 6; Decapentaplegic vegetal related; DVR6; BMP 6; BMP-6; TGFB related vegetal related growth factor; Transforming growth factor beta; Vegetal related TGFB related cytokine; Vg related sequence; VGR1; BMP6_HUMAN.
Background	The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity. [provided by RefSeq].
Application Dilution
WB	1:500-1000
ICC/IF	1:50-100