Filamin A Recombinant Antibody

$Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: Hela; Lane 4: NIH\/3T3; Probed with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: Hela; Lane 4: NIH\/3T3; Probed with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Flow cytometric analysis of Hela cells with Filamin A antibody at 1\/50 dilution (blue) compared with an unlabelled control or cells without incubation with primary antibody (red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.$

Flow cytometric analysis of Hela cells with Filamin A antibody at 1\/50 dilution (blue) compared with an unlabelled control or cells without incubation with primary antibody (red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

$IF(ICC) staining with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:100 in AGS cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:100 in AGS cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse uterus tissue incubated with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse uterus tissue incubated with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52260R
Product Name	Filamin A Recombinant Antibody
Applications	WB FCM IHC-P IF(ICC) IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Filamin A, C-terminus
Clonality	Monoclonal
Clone #	2G3
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	2316
Swiss Prot	P21333
Synonyms	Filamin-A, Actin-binding protein 280, Alpha-filamin, Endothelial actin-binding protein, Filamin-1, Non-muscle filamin, FLN-A, ABP-280
Background	Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs. Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintainance (By similarity).
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC