Active Caspase 3 (2F7) Monoclonal Antibody

$Lane 1: Camptothecin (2 \u00b5M) treated Jurkat cells; Lane 2: Untreated Jurkat cell lysate probed with Active Caspase-3 (2F7) Monoclonal Antibody (bsm-52297R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Camptothecin (2 \u00b5M) treated Jurkat cells; Lane 2: Untreated Jurkat cell lysate probed with Active Caspase-3 (2F7) Monoclonal Antibody (bsm-52297R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-52297R
Product Name	Active Caspase 3 (2F7) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Clonality	Monoclonal
Clone #	2F7
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	836
Swiss Prot	P42574
Synonyms	Caspase-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CASP-3, CPP-32, SCA-1
Background	Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-\|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Application Dilution
WB	1:300-5000