PD-1 Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PD-1 Monoclonal Antibody, Unconjugated (bsm-52367R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PD-1 Monoclonal Antibody, Unconjugated (bsm-52367R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with PD-1 monoclonal antibody, unconjugated (bsm-52367R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with PD-1 monoclonal antibody, unconjugated (bsm-52367R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IF(IHC-P)

Reactivity

Mouse

Overview
Catalog #	bsm-52367r
Product Name	PD-1 Recombinant Antibody
Applications	WB IHC-P IHC-F IF(IHC-P)
Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant mouse PD-1 protein
Clonality	Recombinant
Clone #	3F4
Isotype	IgG1, k
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	18566
Swiss Prot	Q02242
Subcellular location	Cell membrane
Synonyms	ADMIO4; AIMTBS; CD279; PD-1; PD1; SLEB2; hPD-1; hPD-l; hSLE1; Ly101; Pdc1; PDCD1_HUMAN; PDCD1; Protein PD-1; PDCD1_MOUSE; mPD-1; programmed cell death 1; systemic lupus erythematosus susceptibility 2
Background	Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.
Application Dilution
WB	1:500-2000
IHC-P	IHC-P1:100-500
IHC-F	IHC-F1:100-500
IF(IHC-P)	IFIHC-P1:100-500