CAMK2 beta Recombinant Antibody

$Lane 1: SH-SY-5Y cell; Lane 2: PC-12 cell; Lane 3: SHG-44 cell; Probed with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SH-SY-5Y cell; Lane 2: PC-12 cell; Lane 3: SHG-44 cell; Probed with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse cerebellum tissue incubated with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse cerebellum tissue incubated with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat cerebellum tissue incubated with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat cerebellum tissue incubated with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52427R
Product Name	CAMK2 beta Recombinant Antibody
Applications	WB IHC-P IF(ICC) IHC
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human CaMKII between 200-300 amino acids
Clonality	Recombinant
Clone #	1H12
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	816
Swiss Prot	Q13554
Subcellular location	Cytoplasm, Cell membrane
Synonyms	CAM2; CAMK2; CAMKB; CaMKIIbeta; MRD54; CaMKII; Ck2b; KCC2B_HUMAN; CAMK2B; CaM kinase II subunit beta; CaMK-II subunit beta; 2.7.11.17; KCC2B_MOUSE; Camk2d; KCC2B_RAT; calcium/calmodulin dependent protein kinase II beta; calcium/calmodulin-dependent protein kinase (CaM kinase) II beta; CaM-kinase II beta chain; calcium/calmodulin-dependent protein kinase type II beta chain; CaM kinase II beta subunit; proline rich calmodulin-dependent protein kinase
Background	Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC