IL-1 beta Recombinant Antibody

$THP-1 (H) cells were treated with or without TPA (80nM) for 24h,then treated with (100ng\/ml) lipopolysaccharide (LPS) for 6h, then with (300ng\/ml) Brefeldin A added after 3h,25 \u03bcg total protein per lane of cell lysates (see on figure) probed with IL-1 beta monoclonal antibody, unconjugated (bsm-52727R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

THP-1 (H) cells were treated with or without TPA (80nM) for 24h,then treated with (100ng\/ml) lipopolysaccharide (LPS) for 6h, then with (300ng\/ml) Brefeldin A added after 3h,25 \u03bcg total protein per lane of cell lysates (see on figure) probed with IL-1 beta monoclonal antibody, unconjugated (bsm-52727R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-52727r
Product Name	IL-1 beta Recombinant Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within Human IL1 beta aa 100 to the C-terminus
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	3553
Swiss Prot	P01584
Synonyms	Interleukin-1 beta; Catabolin; IL-1 beta; IL1B; IL1F2; IL1-BETA.
Background	Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Application Dilution
WB	1:1000-5000