PAX5 (4C1) Monoclonal Antibody

Flow cytometric analysis of Raji cells with PAX5 (4C1) Monoclonal Antibody (bsm-52735R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Daudi cells Probed with PAX5 (4C1) Monoclonal Antibody (bsm-52735R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Daudi cells Probed with PAX5 (4C1) Monoclonal Antibody (bsm-52735R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with PAX5 (4C1) Monoclonal Antibody (bsm-52735R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with PAX5 (4C1) Monoclonal Antibody (bsm-52735R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with PAX5 (4C1) Monoclonal Antibody (bsm-52735R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with PAX5 (4C1) Monoclonal Antibody (bsm-52735R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with PAX5 (4C1) Monoclonal Antibody (bsm-52735R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with PAX5 (4C1) Monoclonal Antibody (bsm-52735R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
FCM
IF(ICC)
IHC

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52735R
Product Name	PAX5 (4C1) Monoclonal Antibody
Applications	WB FCM IF(ICC) IHC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human PAX5 aa 250-350
Clonality	Monoclonal
Clone #	4C1
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5079
Swiss Prot	Q02548
Synonyms	Paired box protein Pax-5, B-cell-specific transcription factor, BSAP
Background	May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200
IHC