LYVE1 Recombinant Antibody

Flow cytometric analysis of Huvec cells with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: MCF-7 lysates; Probed with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: MCF-7 lysates; Probed with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:100 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:100 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Blood cell lysates; Lane 3: Mouse Lymph node tissue lysates ; Lane 4: Mouse Large intestine tissue lysates ; Lane 5: Rat Thyroid gland tissue lysates ; Lane 6: Rat Spleen tissue lysates ; Lane 7: Rat Lymph node tissue lysates ; Lane 8: Rat Large intestine tissue lysates ; Lane 9: Human Huvec cell lysates ; Lane 10: Human K562 cell lysates probed with LYVE-1 Polyclonal Antibody, Unconjugated (bsm-52811R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n$

Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Blood cell lysates; Lane 3: Mouse Lymph node tissue lysates ; Lane 4: Mouse Large intestine tissue lysates ; Lane 5: Rat Thyroid gland tissue lysates ; Lane 6: Rat Spleen tissue lysates ; Lane 7: Rat Lymph node tissue lysates ; Lane 8: Rat Large intestine tissue lysates ; Lane 9: Human Huvec cell lysates ; Lane 10: Human K562 cell lysates probed with LYVE-1 Polyclonal Antibody, Unconjugated (bsm-52811R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Applications

IHC-P
IHC-F
IF

Reactivity

Human

Overview
Catalog #	bsm-52811r
Product Name	LYVE1 Recombinant Antibody
Applications	IHC-P IHC-F IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human LYVE1 protein
Clonality	Recombinant
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10894
Swiss Prot	Q9Y5Y7
Subcellular location	Cytoplasm, Cell membrane, Extracellular matrix
Synonyms	lymphalic vessel endotheilial hyaluronan receptor 1; CRSBP 1; CRSBP1; extracellular link domain containing 1; extracellular link domain-containing 1; HAR; hyaluronic acid receptor; Cell surface retention sequence-binding protein 1; CRSBP-1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; Lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1_HUMAN; Lymphatic endothelium specific hyaluronan receptor; LYVE 1; LYVE-1; XLKD1.
Application Dilution
IHC-P	1:100-500
IHC-F	1:100-500
IF	1:100-500