Thymidine Kinase 1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Thymidine kinase 1 monoclonal antibody, unconjugated (bsm-52813R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Thymidine kinase 1 monoclonal antibody, unconjugated (bsm-52813R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$The U-2 OS (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Thymidine kinase 1 antibody (bsm-52813R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The U-2 OS (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Thymidine kinase 1 antibody (bsm-52813R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

WB
IHC-P
IP
Flow-Cyt
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-52813r
Product Name	Thymidine Kinase 1 Recombinant Antibody
Applications	WB IHC-P IP FLOW-CYT ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Thymidine Kinase 1 around C terminus
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7083
Swiss Prot	P04183
Synonyms	Thymidine kinase, cytosolic, TK1
Background	Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IP	1-2ug
Flow-Cyt	1ug/Test
ICC/IF	1:50-100