HE4 Recombinant Antibody

Flow cytometric analysis of sw480 cells with HE4 (8C3) Monoclonal Antibody (bsm-52840R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$IF(ICC) staining with HE4 (8C3) Monoclonal Antibody (bsm-52840R) at 1:100 in SKOV-3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with HE4 (8C3) Monoclonal Antibody (bsm-52840R) at 1:100 in SKOV-3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with HE4 (8C3) Monoclonal Antibody (bsm-52840R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with HE4 (8C3) Monoclonal Antibody (bsm-52840R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Human A431 cell lysates probed with HE4 Monoclonal Antibody, Unconjugated (bsm-52840R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Human A431 cell lysates probed with HE4 Monoclonal Antibody, Unconjugated (bsm-52840R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52840r
Product Name	HE4 Recombinant Antibody
Applications	WB IHC-P IHC-F IF FLOW-CYT ICC/IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminal Human HE4
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	10406
Swiss Prot	Q14508
Synonyms	WAP four-disulfide core domain protein 2, Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2
Background	Broad range protease inhibitor.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF
Flow-Cyt	1ug/Test
ICC/IF	1:50-100