AHR Recombinant Antibody

$Lane 1: human lung lysates;Probed with Aryl hydrocarbon Receptor (3C7) Monoclonal Antibody (bsm-52886R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: human lung lysates;Probed with Aryl hydrocarbon Receptor (3C7) Monoclonal Antibody (bsm-52886R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Aryl hydrocarbon Receptor (3C7) Monoclonal Antibody (bsm-52886R) at 1:200 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Aryl hydrocarbon Receptor (3C7) Monoclonal Antibody (bsm-52886R) at 1:200 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Aryl hydrocarbon Receptor (3C7) Monoclonal Antibody (bsm-52886R) at 1:200 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Aryl hydrocarbon Receptor (3C7) Monoclonal Antibody (bsm-52886R) at 1:200 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Aryl hydrocarbon Receptor (3C7) Monoclonal Antibody (bsm-52886R) at 1:200 in NIH-3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Aryl hydrocarbon Receptor (3C7) Monoclonal Antibody (bsm-52886R) at 1:200 in NIH-3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52886r
Product Name	AHR Recombinant Antibody
Applications	WB IHC-P IF(ICC)
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Aryl hydrocarbon Receptor aa 600-800
Clonality	Recombinant
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	196
Swiss Prot	P35869
Synonyms	Class E basic helix-loop-helix protein 76, Ah receptor, AhR, bHLHe76
Background	Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates (PubMed:10395741, PubMed:28602820, PubMed:7961644, PubMed:23275542, PubMed:30373764). Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene) (PubMed:7961644). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons (PubMed:7961644). Involved in cell-cycle regulation (PubMed:12213388). Likely to play an important role in the development and maturation of many tissues (PubMed:12213388). Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1 (PubMed:28602820). Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1. The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28602820).
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200