EDG1 Recombinant Antibody

Flow cytometric analysis of Jurkat cells with EDG1 (1C1) Monoclonal Antibody (bsm-52902R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$25 ug total protein per lane of various lysates (see on figure) probed with EDG1 monoclonal antibody, unconjugated (bsm-52902R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with EDG1 monoclonal antibody, unconjugated (bsm-52902R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human

Predicted Reactivity

Overview
Catalog #	bsm-52902r
Product Name	EDG1 Recombinant Antibody
Applications	WB FCM IHC-P IF(ICC) IHC
Reactivity	Human
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human EDG1 aa 303-382
Immunogen Range	2-51/382
Clonality	Recombinant
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1901
Swiss Prot	P21453
Synonyms	Sphingosine 1-phosphate receptor 1, Endothelial differentiation G-protein coupled receptor 1, Sphingosine 1-phosphate receptor Edg-1, S1P receptor 1, S1P1, S1P receptor Edg-1, S1PR1
Background	This gene encodes a protein that may regulate endothelial cell differentiation, lipid metabolism, and hormone-induced cardiomyocyte hypertrophy. The encoded protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC