SirT1 (9C9) Monoclonal Antibody

$Western blot was performed using nuclear extracts from HeLa cells (20 \u03bcg) and SirT1 (9C9) Monoclonal Antibody (bsm-53011M) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left, the position of the protein of interest is shown on the right.$

Western blot was performed using nuclear extracts from HeLa cells (20 \u03bcg) and SirT1 (9C9) Monoclonal Antibody (bsm-53011M) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left, the position of the protein of interest is shown on the right.

Applications

Reactivity

Human

Overview
Catalog #	bsm-53011M
Product Name	SirT1 (9C9) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Monoclonal antibody raised in mouse against human SirT1 (NAD-dependent deacetylase sirtuin-1) using a synthetic peptide
Clonality	Monoclonal
Clone #	9C9
Isotype	IgG1
Concentration	0.7ug/ul
Purification	Protein G purified monoclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	23411
Swiss Prot	Q96EB6
Synonyms	SIR2, SIR2alpha, SIR2L1
Background	This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
Application Dilution
WB	1:300-5000