phospho-MLKL (Ser345) Recombinant Antibody

$4% Paraformaldehyde-fixed L-929\uff08treated with 20 ng\/ml TNF alpha, 100 nM Smac mimetic, and 20 \u00b5M z-VAD for 8 h\uff09 (M) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-MLKL (Ser345)) monoclonal Antibody, unconjugated (bsm-54104R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed L-929\uff08treated with 20 ng\/ml TNF alpha, 100 nM Smac mimetic, and 20 \u00b5M z-VAD for 8 h\uff09 (M) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-MLKL (Ser345)) monoclonal Antibody, unconjugated (bsm-54104R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Applications

WB
IP
ICC/IF

Reactivity

Mouse

Overview
Catalog #	bsm-54104r
Product Name	phospho-MLKL (Ser345) Recombinant Antibody
Applications	WB IP ICC/IF
Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to residues surrounding Ser345 of human MLKL
Modification Site	Ser345
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	74568
Swiss Prot	Q9D2Y4
Synonyms	Phospho-MLKL (S345); pMLKL Ser345; Mixed lineage kinase domain-like protein, Mlkl
Background	Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.
Application Dilution
WB	1:300-5000
IP	1-2ug
ICC/IF	1:50-100