FYB1 (1F10) Monoclonal Antibody

$Lane 1: U937 Cells; Probed with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: U937 Cells; Probed with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

$Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.$

Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

$Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.$

Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Flow cytometric analysis of HepG2 cells with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IHC-P
IHC

Reactivity

Human

Overview
Catalog #	bsm-54461R
Product Name	FYB1 (1F10) Monoclonal Antibody
Applications	WB FCM IHC-P IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within C-terminal human FYB.
Clonality	Monoclonal
Clone #	1F10
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	2533
Swiss Prot	O15117
Subcellular location	Cytoplasm, Nucleus, Cell junction
Synonyms	FYN-binding protein 1; Adhesion and degranulation promoting adaptor protein; ADAP; FYB-120/130; p120/p130; FYN-T-binding protein; SLAP-130; SLP-76-associated phosphoprotein; SLAP130; FYB
Background	Acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2. May play a role in linking T-cell signaling to remodeling of the actin cytoskeleton.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC