Phospho-IRF3(S386) Monoclonal Antibody

$MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-IRF3(S386)) Monoclonal Antibody, Unconjugated (bsm-54508R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.$

MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-IRF3(S386)) Monoclonal Antibody, Unconjugated (bsm-54508R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Applications

WB
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-54508R
Product Name	Phospho-IRF3(S386) Monoclonal Antibody
Applications	WB IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-Peptide corresponding to residues surrounding Ser386 of human IRF3.
Modification Site	Ser386
Clonality	Monoclonal
Clone #	6H4
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	3661
Swiss Prot	Q14653
Subcellular location	Cytoplasm, Nucleus
Synonyms	IIAE7 antibody, Interferon regulatory factor 3 antibody, IRF 3 antibody, IRF-3 antibody, IRF3 antibody, IRF3_HUMAN antibody, MGC94729 antibody
Background	This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011].
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200