HDAC3 Monoclonal Antibody

$The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature.Primary Antibody (green):Mouse Anti-HDAC3 antibody (bsm-60742M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature.Primary Antibody (green):Mouse Anti-HDAC3 antibody (bsm-60742M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

Reactivity

Human

Overview
Catalog #	bsm-60742m
Product Name	HDAC3 Monoclonal Antibody
Applications	WB FCM
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human HDAC3
Clonality	Monoclonal
Clone #	A11D3
Isotype	IgG2b, Kappa
Concentration	Lot Dependent
Purification	Purified by Protein G.
Storage Buffer	PBS, Glycerol, BSA.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8841
Swiss Prot	O15379
Subcellular location	Nucleus, Cell membrane
Synonyms	RPD3 2; RPD3-2; HD 3; HD3; HDAC 3; Histone deacetylase 3(HD3)(RPD3-2); histone deacetylase 3; RPD 3; RPD3; SMAP 45; SMAP-45; SMAP45; HDAC3_HUMAN
Application Dilution
WB	1:500-1000
FCM	FCM1:50-100