ACTH Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Human pituitary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACTH Monoclonal Antibody, Unconjugated(bsm-60798R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human pituitary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACTH Monoclonal Antibody, Unconjugated(bsm-60798R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with ACTH monoclonal antibody, unconjugated (bsm-60798R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ACTH monoclonal antibody, unconjugated (bsm-60798R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Rat pituitary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTH Monoclonal Antibody, Unconjugated (bsm-60798R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat pituitary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ACTH Monoclonal Antibody, Unconjugated (bsm-60798R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Mouse

Overview
Catalog #	bsm-60798r
Product Name	ACTH Recombinant Antibody
Applications	WB IHC-P IHC-F IF(IHC-P)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ACTH
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	PBS, Glycerol, BSA.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Nucleus
Synonyms	Adrenocorticotropic hormone; Adrenocorticotropin; Adrenocorticotropin Hormone; alpha Melanocyte Stimulating Hormone; beta Lipotropin; beta Melanocyte Stimulating Hormone; CLIP; Corticotropin; Corticotropin Like Intermediary Peptide; Corticotropin lipotropin precursor; Lipotropin beta; Lipotropin gamma; LPH; Melanotropin alpha; Met Enkephalin; MSH; NPP; POC; POMC; Pro opiomelanocortin; Proopiomelanocortin; COLI_HUMAN.
Application Dilution
WB	1:500-2000
IHC-P	IHC-P1:50-200
IHC-F	IHC-F1:50-200
IF(IHC-P)	IFIHC-P1:50-200