Occludin Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Occludin monoclonal antibody, unconjugated (bsm-61062R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Occludin monoclonal antibody, unconjugated (bsm-61062R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bsm-61062r
Product Name	Occludin Recombinant Antibody
Applications	WB FCM
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse OCLN
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	18260
Swiss Prot	Q61146
Subcellular location	Cell membrane
Synonyms	OCLN_HUMAN; Ocl; BLCPMG; PPP1R115; PTORCH1; Tight junction protein occludin;
Background	This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq].
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100