RNA polymerase beta Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with RNA polymerase beta monoclonal antibody, unconjugated (bsm-61640R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with RNA polymerase beta monoclonal antibody, unconjugated (bsm-61640R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Escherichia Coli

Overview
Catalog #	bsm-61640r
Product Name	RNA polymerase beta Recombinant Antibody
Applications	WB IP
Reactivity	Escherichia Coli
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from RNA polymerase beta
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	86861612
Swiss Prot	P0A8V2
Synonyms	groN; nitB; rif; RNA pol beta; RNA polymerase subunit beta; ron; rpoB; stl; stv; tabD; Transcriptase subunit beta.
Background	DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Resistance to the antibiotics salinamide A, salinamide B, rifampicin, streptolydigin, CBR703, myxopyronin, and lipiarmycin can result from mutations in this protein.
Application Dilution
WB	1:500-2000
IP	IP1:20-50