Phosphoserine/threonine Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70605m |
| Product Name | Phosphoserine/threonine Antibody |
| Applications |
WB
IP
|
| Specificity | The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Phosphoserine/threonine is a mix of Clone psA and clone psB. They were generated from synthetic peptides of phosphoserine and phosphothreonine (coupled to carrier protein), respectively. |
| Modification Site | pSer/Thr |
| Clonality | Monoclonal |
| Clone # | psA/psB |
| Isotype | IgG1 |
| Purification | Purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | N/A |
| Synonyms | Phosphoser/thr mAb |
| Background | Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity. |
| Application Dilution | |
| WB | 1:200-500 |
| IP | 1:50 |