PTP1B (C-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70631M |
| Product Name | PTP1B (C-terminal region) Antibody |
| Applications |
WB
|
| Specificity | This antibody detects a 50 kDa* band corresponding to PTP1B in western blots of human A431, Jurkat, and HeLa cells. It does not detect PTP1B in rat PC12 or mouse SYF cells. |
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone M234 was generated from a recombinant protein corresponding to amino acids in the C-terminal region of human PTP1B. |
| Clonality | Monoclonal |
| Clone # | M234 |
| Isotype | IgG2a |
| Purification | Purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C. |
| Target | |
| Swiss Prot | P18031 |
| Synonyms | PTPN1; TCPTP/PTPN2 |
| Background | PTP1B is a nonreceptor type protein tyrosine phosphatase that has essential roles in insulin and leptin signaling, as well as important functions in growth factor and integrin signaling. The structure of PTP1B includes a conserved phosphatase domain, C-terminal hydrophobic residues for targeting to the cytoplasmic face of the endoplasmic reticulum, and proline-rich regions characteristic of SH3 domain binding motifs. PTP1B can interact with N-Cadherin and dephosphorylate _-catenin associated with cadherin complexes. PTP1B also interacts with Insulin and EGF receptors, and undergoes phosphorylation after receptor stimulation. Tyrosine phosphorylation at Tyr-66, Tyr-152, and Tyr-153 occurs after insulin receptor activation, and tyrosine phosphorylation of Tyr-152 may be required for interactions with N-Cadherin. In addition, Akt can phosphorylate Ser-50 and this phosphorylation can reduce PTP1B activity. |
| Application Dilution | |
| WB | 1:300-5000 |