ABCG2 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti ABCG2\/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti ABCG2\/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti ABCG2\/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti ABCG2\/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin-embedded rat brain labeled with Anti-ABCG2\/CD338 Polyclonal Antibody, Unconjugated(bs-0662R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.$

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-ABCG2\/CD338 Polyclonal Antibody, Unconjugated(bs-0662R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

$L1 rat brain lysates L2 mouse embryo lysates probed with Anti ABCG2\/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 72kD. Observed band size:72kD, 28kD.\\n$

L1 rat brain lysates L2 mouse embryo lysates probed with Anti ABCG2\/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 72kD. Observed band size:72kD, 28kD.\\n

$Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Lung tissue lysates; Lane 4: Rat Placenta tissue lysates; Lane 5: Rat Cerebrum tissue lysates; Lane 6: Rat Lung tissue lysates; Lane 7: Human MCF-7 cell lysates; Lane 8: Human HepG2 cell lysates; Lane 9: Human HUVEC cell lysates probed with ABCG2 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Lung tissue lysates; Lane 4: Rat Placenta tissue lysates; Lane 5: Rat Cerebrum tissue lysates; Lane 6: Rat Lung tissue lysates; Lane 7: Human MCF-7 cell lysates; Lane 8: Human HepG2 cell lysates; Lane 9: Human HUVEC cell lysates probed with ABCG2 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-0662r
Product Name	ABCG2 Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ABCG2
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9429
Swiss Prot	Q9UNQ0
Subcellular location	Cell membrane
Synonyms	ABC15; ABCP; BCRP; BCRP1; BMDP; CD338; CDw338; CDw388; EST157481; GOUT1; MRX; MXR; MXR-1; MXR1; UAQTL1; ABCG2; ABCG2_HUMAN; ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; Urate exporter; 7.6.2.2; ABCG2_MOUSE; Breast cancer resistance protein 1 homolog; ABCG2_PIG; Brain multidrug resistance protein; ABCG2_RAT; ATP binding cassette subfamily G member 2 (JR blood group); ATP-binding cassette, sub-family G (WHITE), member 2; ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group); ATP binding cassette subfamily G member 2 (Junior blood group)
Application Dilution
WB	=1:500-2000
ELISA	ELISA=1:5000-10000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:100-500
IF	IF=1:100-500