Principle of the Assay
The kit uses enzyme-linked immunoassay. According to the structural characteristics of IgM antibodies, the same protein antigen was selected to coat the microporous plate and to be labeled with horseradish peroxidase (HRP); Labeled SARS-CoV-2-n recombinant protein (labeled antigen) with horseradish peroxidase (HRP). The kit design is as follows:
The 96-well microporous plate is coated with a recombinant SARS-CoV-2-n protein (solid-phase antigen); When the positive sample is to be tested (IgM) and the labeled antigen is added to the microplate, the pattern and product of (solid antigen +IgMAb+ labeled antigen) → (solid antigen -IgMAb-4 labeled antigen) will be formed. Absorbance was measured at 450nm wavelength by a microplate reader. The concentration of IgM antibody in the sample is directly proportional to the absorbance (OD value).
Data Analysis Assistance
We have partnered with MyAssays to offer you an easy to use and versatile tool to analyze the data you receive using our ELISA Kit. Click the link below to be directed to the data analysis tool provided by MyAssays specifically for bskv0002.