α-Glucosidase (α-GC) Activity Assay Kit

Overview

This kit measures α-glucosidase enzymatic activity—how effectively the enzyme hydrolyzes its assay substrate—rather than the amount of α-glucosidase protein present. Activity is determined from the formation of p-nitrophenol as the enzyme breaks down p-nitrophenyl-α-D-glucopyranoside.

α-GC (EC 3.2.1.20) is widely found in animals, plants, microorganisms, and cultured cells. It catalyzes the hydrolysis of α-glycosidic bonds between aryl or alkyl groups and glycosyl groups to produce glucose. This enzyme is not only involved in the loosening or reinforcement of cell walls but also closely related to cell recognition and the production of certain signaling molecules.

Assay principle

α-GC hydrolyzes p-nitrophenyl-α-D-glucopyranoside to produce p-nitrophenol, which has a maximum absorption peak at 400nm. The activity of α-GC is calculated by measuring the rate of increase in absorbance.