Pyruvate (PA) Content Assay Kit

Overview

This kit measures pyruvate content rather than the activity of enzymes that produce or consume pyruvate. Pyruvate is quantified through a color-forming reaction with 2,4-dinitrophenylhydrazine.

Pyruvate serves as a critical metabolic hub, linking glucose, fatty acid, and amino acid metabolism through acetyl-CoA.

Assay principle

Pyruvate reacts with 2,4-dinitrophenylhydrazine to form pyruvate-2,4-dinitrophenylhydrazone, which appears cherry-red in alkaline solution.