Lactate Dehydrogenase (LDH) Activity Assay Kit

Overview

This kit measures lactate dehydrogenase enzymatic activity rather than the amount of LDH protein present. It evaluates the enzyme-catalyzed conversion of lactate to pyruvate, using a colorimetric measurement of the pyruvate produced.

Lactate dehydrogenase (LDH, EC 1.1.1.27) is widely present in animals, plants, microorganisms, and cultured cells. As the terminal enzyme of glycolysis, LDH catalyzes the reversible conversion between pyruvate and lactate, accompanied by the interconversion of NAD+/NADH.

Assay principle

LDH oxidizes lactate to pyruvate using NAD+. Pyruvate reacts with 2,4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which exhibits a brown-red color in alkaline solution. The color intensity is proportional to pyruvate concentration.