Soil β-Glucosidase (S-β-GC) Activity Assay Kit

Overview

This kit measures soil β-glucosidase enzymatic activity rather than the amount of enzyme protein present. Activity is assessed by measuring p-nitrophenol formed when the enzyme hydrolyzes p-nitrophenyl-β-D-glucopyranoside.

β-Glucosidase(β-D-Glucosidase,S-β-GC,EC3.2.1.21),also known as β-D-glucoside glucohydrolase, gentiobiase, cellobiase (CB or β-G), and amygdalase, belongs to the cellulase family. It is a key component of the cellulose-degrading enzyme system, capable of hydrolyzing terminal non-reducing β-D-glucosidic bonds to release β-D-glucose and corresponding aglycones. S-β-GC plays a vital physiological role in the carbohydrate metabolism of soil microorganisms.

Assay principle

S-β-GC catalyzes the hydrolysis of p-nitrophenyl-β-D-glucopyranoside to produce p-nitrophenol, which exhibits characteristic absorption at 400 nm.