ACTH (7-23) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0004r-cy5-100uL |
| Product Name | ACTH (7-23) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ACTH |
| Immunogen Range | 7-23/39 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5443 |
| Swiss Prot | P01189 |
| Subcellular location | Secreted |
| Synonyms | Adrenocorticotropic hormone; Adrenocorticotropin; Adrenocorticotropin Hormone; Alpha Melanocyte Stimulating Hormone; Beta Endorphin; Beta Lipotropin; Beta Melanocyte Stimulating Hormone; CLIP; Corticotropin; Corticotropin Like Intermediary Peptide; Corticotropin lipotropin precursor; Lipotropin Beta; Lipotropin Gamma; LPH; Melanotropin Alpha; Met Enkephalin; MSH; NPP; POC; POMC; Pro opiomelanocortin; Proopiomelanocortin. |
| Background | ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:100-500 |
| IF(ICC) | 1:200-400 |
Frequently asked questions
Which applications is ACTH (7-23) Polyclonal Antibody, Cy5 Conjugated (bs-0004R-Cy5) validated for, and at what dilution?
bs-0004R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:100-500); IF(ICC) (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ACTH (7-23) Polyclonal Antibody, Cy5 Conjugated (bs-0004R-Cy5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Rabbit, GuineaPig.
How should bs-0004R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ACTH (7-23) Polyclonal Antibody, Cy5 Conjugated (bs-0004R-Cy5)?
KLH conjugated synthetic peptide derived from human ACTH Immunogen range: 7-23/39.
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-0004R-Cy5)?
Cy5 on bs-0004R-Cy5: excitation / emission 625,650nm/670nm.