IGF 1 Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0014R-PE-100uL |
| Product Name | IGF 1 Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Dog |
| Predicted Reactivity | Cow, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IGF-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3479 |
| Swiss Prot | P05019 |
| Subcellular location | Secreted |
| Synonyms | IGFI; IGF-I; IGF1A; Insulin-like growth factor I; Mechano growth factor; MGF; Somatomedin-C; IGF1; IBP1 |
| Background | The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is IGF 1 Polyclonal Antibody, PE Conjugated (bs-0014R-PE) validated for, and at what dilution?
bs-0014R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IGF 1 Polyclonal Antibody, PE Conjugated (bs-0014R-PE) react with?
Tested reactivity: Human, Mouse, Rat, Dog. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig, Rabbit.
How should bs-0014R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IGF 1 Polyclonal Antibody, PE Conjugated (bs-0014R-PE)?
KLH conjugated synthetic peptide derived from human IGF-1
What are the excitation and emission wavelengths of the PE conjugate (bs-0014R-PE)?
PE on bs-0014R-PE: excitation / emission 496,564nm/578nm.