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BTG2 Polyclonal Antibody, AbBy Fluor-680 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Pig
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-0031R-BF680-100uL
Product Name BTG2 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Pig, Rabbit, GuineaPig
Specifications
Conjugation AbBy Fluor-680
Host Rabbit
Source KLH conjugated synthetic peptide derived from human BTG2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7832
Swiss Prot P78543
Subcellular location Secreted
Synonyms BTG2_HUMAN; Protein BTG2; BTG family member 2; NGF-inducible anti-prolerative protein PC3; pheochromacytoma cell-3; nerve growth factor-inducible anti-prolerative; B-cell translocation gene 2; BTG2; TIS21; PC3; TIS21.
Background BTG2 is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. BTG2 is involved in the regulation of the G1/S transition of the cell cycle. It modulates transcription regulation mediated by ESR1(referenced from Entrez Gene). BTG2 expression is induced in vivo during neurogenesis, and the gene is transiently expressed in vitro in rat pheochromocytoma PC12 cells after induction of neuronal differentiation by addition of nerve growth factor (NGF); suggesting that BTG2 is functionally significant during the neuronal differentiation process (PMID: 12360398).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is BTG2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0031R-BF680) validated for, and at what dilution?

bs-0031R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BTG2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0031R-BF680) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Pig, Rabbit, GuineaPig.

How should bs-0031R-BF680 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BTG2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0031R-BF680)?

KLH conjugated synthetic peptide derived from human BTG2

What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-0031R-BF680)?

AbBy Fluor-680 on bs-0031R-BF680: excitation / emission 679nm/702nm.

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