phospho-CREB-1 (Ser133) Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0036R-PE |
| Product Name | phospho-CREB-1 (Ser133) Polyclonal Antibody, PE Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Chicken |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CREB-1 around the phosphorylation site of Ser133 [RP(p-S)YR] |
| Modification Site | Ser133 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1385 |
| Swiss Prot | P16220 |
| Subcellular location | Nucleus |
| Synonyms | CREB; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1; CREB1 |
| Background | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is phospho-CREB-1 (Ser133) Polyclonal Antibody, PE Conjugated (bs-0036R-PE) validated for, and at what dilution?
bs-0036R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-CREB-1 (Ser133) Polyclonal Antibody, PE Conjugated (bs-0036R-PE) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Chicken.
How should bs-0036R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CREB-1 (Ser133) Polyclonal Antibody, PE Conjugated (bs-0036R-PE)?
KLH conjugated synthetic phosphopeptide derived from human CREB-1 around the phosphorylation site of Ser133 [RP(p-S)YR]
What are the excitation and emission wavelengths of the PE conjugate (bs-0036R-PE)?
PE on bs-0036R-PE: excitation / emission 496,564nm/578nm.