ChAT Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0042R-BF594-100uL |
| Product Name | ChAT Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ChAT |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1103 |
| Swiss Prot | P28329 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CMS1A; CMS1A2; CHOACTASE; Choline O-acetyltransferase; ChAT; Choline acetylase |
| Background | Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ChAT Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-0042R-BF594) validated for, and at what dilution?
bs-0042R-BF594 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ChAT Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-0042R-BF594) react with?
Tested reactivity: Human.
How should bs-0042R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ChAT Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-0042R-BF594)?
KLH conjugated synthetic peptide derived from human ChAT
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-0042R-BF594)?
AbBy Fluor-594 on bs-0042R-BF594: excitation / emission 590nm/617nm.