DFFB Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0043R-RBITC |
| Product Name | DFFB Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Rabbit, GuineaPig |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse DFF-40 beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 13368 |
| Swiss Prot | O54788 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik; DNA fragmentation factor subunit beta; Caspase-activated deoxyribonuclease; Caspase-activated DNase; DNA fragmentation factor 40 kDa subunit; DFF-40; Dffb |
| Background | Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DFFB Polyclonal Antibody, RBITC Conjugated (bs-0043R-RBITC) validated for, and at what dilution?
bs-0043R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DFFB Polyclonal Antibody, RBITC Conjugated (bs-0043R-RBITC) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Rabbit, GuineaPig.
How should bs-0043R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DFFB Polyclonal Antibody, RBITC Conjugated (bs-0043R-RBITC)?
KLH conjugated synthetic peptide derived from mouse DFF-40 beta
What are the excitation and emission wavelengths of the RBITC conjugate (bs-0043R-RBITC)?
RBITC on bs-0043R-RBITC: excitation / emission 540nm/625nm.