Caspase-9 Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0050R-PE |
| Product Name | Caspase-9 Polyclonal Antibody, PE Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Caspase-9 subunit p35 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 842 |
| Swiss Prot | P55211 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6; CASP9 |
| Background | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is Caspase-9 Polyclonal Antibody, PE Conjugated (bs-0050R-PE) validated for, and at what dilution?
bs-0050R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Caspase-9 Polyclonal Antibody, PE Conjugated (bs-0050R-PE) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig, Rabbit.
How should bs-0050R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Caspase-9 Polyclonal Antibody, PE Conjugated (bs-0050R-PE)?
KLH conjugated synthetic peptide derived from human Caspase-9 subunit p35
What are the excitation and emission wavelengths of the PE conjugate (bs-0050R-PE)?
PE on bs-0050R-PE: excitation / emission 496,564nm/578nm.