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    Caspase 8 Polyclonal Antibody

    Description:
    Applications:
    • WB
    • IHC-P
    • IF(IHC-P)
    • IF(ICC)
    Overview
    Catalog # bs-0052R
    Product Name Caspase 8 Polyclonal Antibody
    Applications WB, IHC-P, IF(IHC-P), IF(ICC)
    Reactivity Human, Mouse, Rat
    Specifications
    Conjugation Unconjugated
    Host Rabbit
    Source KLH conjugated synthetic peptide derived from mouse Caspase-8 subunit p10
    Immunogen Range 430-482/482
    Clonality Polyclonal
    Isotype IgG
    Concentration 1ug/ul
    Purification Purified by Protein A.
    Storage Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
    Target
    Gene ID 12370
    Swiss Prot O89110
    Subcellular location Cytoplasm
    Synonyms MACH; Mch5; FLICE; CASP-8; Caspase-8; Casp8
    Background Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP1. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.
    Applications
    Application Dilution
    WB 1:100-1000
    IHC-P 1:100-500
    IF(IHC-P) 1:50-200
    IF(ICC) 1:50-200