APAF1(NT) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0059R-RBITC-100uL |
| Product Name | APAF1(NT) Polyclonal Antibody, RBITC Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Apaf-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 317 |
| Swiss Prot | O14727 |
| Subcellular location | Cytoplasm |
| Synonyms | CED4; APAF-1; Apoptotic protease-activating factor 1; APAF1; KIAA0413 |
| Background | Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is APAF1(NT) Polyclonal Antibody, RBITC Conjugated (bs-0059R-RBITC) validated for, and at what dilution?
bs-0059R-RBITC is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does APAF1(NT) Polyclonal Antibody, RBITC Conjugated (bs-0059R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Horse, Chicken.
How should bs-0059R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise APAF1(NT) Polyclonal Antibody, RBITC Conjugated (bs-0059R-RBITC)?
KLH conjugated synthetic peptide derived from human Apaf-1
What are the excitation and emission wavelengths of the RBITC conjugate (bs-0059R-RBITC)?
RBITC on bs-0059R-RBITC: excitation / emission 540nm/625nm.