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Substance P Polyclonal Antibody, PE-Cy5 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
  • Rat
  • GuineaPig

Predicted Reactivity

  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-0065R-PE-Cy5
Product Name Substance P Polyclonal Antibody, PE-Cy5 Conjugated
Applications
WB FCM
Reactivity Human, Mouse, Rat, GuineaPig
Predicted Reactivity Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation PE-Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SP
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6863
Swiss Prot P20366
Subcellular location Secreted
Synonyms NK2; NPK; NKNA; TAC2; Hs.2563; Protachykinin-1; PPT; TAC1; NKA
Background Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
Application Dilution
WB 1:300-5000
FCM 1:20-100

Frequently asked questions

Which applications is Substance P Polyclonal Antibody, PE-Cy5 Conjugated (bs-0065R-PE-Cy5) validated for, and at what dilution?

bs-0065R-PE-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Substance P Polyclonal Antibody, PE-Cy5 Conjugated (bs-0065R-PE-Cy5) react with?

Tested reactivity: Human, Mouse, Rat, GuineaPig. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig, Horse, Rabbit.

How should bs-0065R-PE-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Substance P Polyclonal Antibody, PE-Cy5 Conjugated (bs-0065R-PE-Cy5)?

KLH conjugated synthetic peptide derived from human SP

What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-0065R-PE-Cy5)?

PE-Cy5 on bs-0065R-PE-Cy5: excitation / emission 488nm/670nm.