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Substance P Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
  • GuineaPig

Predicted Reactivity

  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-0065R-PE
Product Name Substance P Polyclonal Antibody, PE Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat, GuineaPig
Predicted Reactivity Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SP
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6863
Swiss Prot P20366
Subcellular location Secreted
Synonyms NK2; NPK; NKNA; TAC2; Hs.2563; Protachykinin-1; PPT; TAC1; NKA
Background Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is Substance P Polyclonal Antibody, PE Conjugated (bs-0065R-PE) validated for, and at what dilution?

bs-0065R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Substance P Polyclonal Antibody, PE Conjugated (bs-0065R-PE) react with?

Tested reactivity: Human, Mouse, Rat, GuineaPig. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig, Horse, Rabbit.

How should bs-0065R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Substance P Polyclonal Antibody, PE Conjugated (bs-0065R-PE)?

KLH conjugated synthetic peptide derived from human SP

What are the excitation and emission wavelengths of the PE conjugate (bs-0065R-PE)?

PE on bs-0065R-PE: excitation / emission 496,564nm/578nm.