TGF alpha Polyclonal Antibody

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat colon labeled with Anti-TGF alpha Polyclonal Antibody Unconjugated (bs-0066R) followed by conjugation to the secondary antibody and DAB staining

Hela cells probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) for 30 minutes followed by incubation with a conjugated secondary (FITC conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Colon tissue lysates; Lane 3: Mouse Spleen tissue lysates; Lane 4: Rat Kidney tissue lysates; Lane 5: Rat Liver tissue lysates; Lane 6: Rat Spleen tissue lysates; Lane 7: Human Jurkat cell lysates; Lane 8: Human THP-1 cell lysates; Lane 9: Human A431 cell lysates; Lane 10: Human HepG2 cell lysates; Lane 11: Human MCF-7 cell lysates; Lane 12: Human U87MG cell lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Rat Kidney tissue lysates; Lane 4: Rat Liver tissue lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Rat Kidney tissue lysates; Lane 2: Rat Liver tissue lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.