MAP3K14/NFkB Inducing Kinase Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0074R-RBITC |
| Product Name | MAP3K14/NFkB Inducing Kinase Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NIK |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9020 |
| Swiss Prot | Q99558 |
| Subcellular location | Cytoplasm |
| Synonyms | HS; NIK; HSNIK; FTDCR1B; Mitogen-activated protein kinase kinase kinase 14; NF-kappa-beta-inducing kinase; Serine/threonine-protein kinase NIK; MAP3K14 |
| Background | Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P1, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MAP3K14/NFkB Inducing Kinase Polyclonal Antibody, RBITC Conjugated (bs-0074R-RBITC) validated for, and at what dilution?
bs-0074R-RBITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MAP3K14/NFkB Inducing Kinase Polyclonal Antibody, RBITC Conjugated (bs-0074R-RBITC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Horse, Rabbit.
How should bs-0074R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MAP3K14/NFkB Inducing Kinase Polyclonal Antibody, RBITC Conjugated (bs-0074R-RBITC)?
KLH conjugated synthetic peptide derived from human NIK
What are the excitation and emission wavelengths of the RBITC conjugate (bs-0074R-RBITC)?
RBITC on bs-0074R-RBITC: excitation / emission 540nm/625nm.