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Nociceptin Polyclonal Antibody, AbBy Fluor-488 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-0075R-BF488-100uL
Product Name Nociceptin Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation AbBy Fluor-488
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Nociceptin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5368
Swiss Prot Q13519
Subcellular location Secreted
Synonyms PPNOC; Prepronociceptin; PNOC; OFQ
Background Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development (By similarity).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Nociceptin Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0075R-BF488) validated for, and at what dilution?

bs-0075R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Nociceptin Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0075R-BF488) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse, Rabbit.

How should bs-0075R-BF488 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Nociceptin Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0075R-BF488)?

KLH conjugated synthetic peptide derived from human Nociceptin

What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-0075R-BF488)?

AbBy Fluor-488 on bs-0075R-BF488: excitation / emission 499nm/519nm.

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