VIP Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0077R-BF594 |
| Product Name | VIP Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VIP |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7432 |
| Swiss Prot | P01282 |
| Subcellular location | Secreted |
| Synonyms | Vasoactive Intestinal peptide; MGC13587; Peptide histidine methioninamide 27; PHM 27; PHM27; PHV42; VIP peptides precursor; PHI; Peptide Histidine Isoleucine; Vasoactive intestinal peptide. |
| Background | VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. PHM and PHV also cause vasodilation. PHM-27 is a potent agonist of the calcitonin receptor CALCR, with similar efficacy as calcitonin. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is VIP Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-0077R-BF594) validated for, and at what dilution?
bs-0077R-BF594 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VIP Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-0077R-BF594) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Chicken.
How should bs-0077R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VIP Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-0077R-BF594)?
KLH conjugated synthetic peptide derived from human VIP
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-0077R-BF594)?
AbBy Fluor-594 on bs-0077R-BF594: excitation / emission 590nm/617nm.