TNF alpha Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0078R-BF350-100uL |
| Product Name | TNF alpha Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Dog, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TNF alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7124 |
| Swiss Prot | P01375 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | DIF; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; Cachectin; Tumor necrosis factor ligand superfamily member 2; TNF-a; TNF |
| Background | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TNF alpha Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-0078R-BF350) validated for, and at what dilution?
bs-0078R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TNF alpha Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-0078R-BF350) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Pig, Horse.
How should bs-0078R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TNF alpha Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-0078R-BF350)?
KLH conjugated synthetic peptide derived from human TNF alpha
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-0078R-BF350)?
AbBy Fluor-350 on bs-0078R-BF350: excitation / emission 346nm/442nm.