CD20 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0080R-APC-Cy7 |
| Product Name | CD20 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cat |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD20 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 931 |
| Swiss Prot | P11836 |
| Subcellular location | Cell membrane |
| Synonyms | B1; S7; Bp35; CD2; CVID5; MS4A2; LEU-16; B-lymphocyte antigen CD2; B-lymphocyte surface antigen B1; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; MS4A1 |
| Background | B lymphocytes marker CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CD20 Polyclonal Antibody, APC-Cy7 Conjugated (bs-0080R-APC-Cy7) validated for, and at what dilution?
bs-0080R-APC-Cy7 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD20 Polyclonal Antibody, APC-Cy7 Conjugated (bs-0080R-APC-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cat.
How should bs-0080R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD20 Polyclonal Antibody, APC-Cy7 Conjugated (bs-0080R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human CD20
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-0080R-APC-Cy7)?
APC-Cy7 on bs-0080R-APC-Cy7: excitation / emission 650nm/780nm.