MUC16 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MUC16 Polyclonal Antibody, Unconjugated (bs-0091R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MUC16 Polyclonal Antibody, Unconjugated (bs-0091R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Applications

IHC-P
IF(IHC-P)

Reactivity

Human

Overview
Catalog #	bs-0091R
Product Name	MUC16 Polyclonal Antibody
Applications	IHC-P, IF(IHC-P)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CA125
Immunogen Range	12760-12810/22152
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Target
Gene ID	94025
Swiss Prot	Q8WXI7
Subcellular location	Extracellular
Synonyms	CA125; Mucin-16; MUC-16; Ovarian cancer-related tumor marker CA125; CA-125; Ovarian carcinoma antigen CA125; MUC16
Background	Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Application Dilution
IHC-P	1:200-400
IF(IHC-P)	1:50-200

Applications

Reactivity

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